"Induction of pluripotent
stem cells from adult human fibroblasts by defined
factors," by Kazutoshi Takahashi, Koji Tanabe, Mari Ohnuki,
Megumi Narita, Tomoko Ischisaka, Kiichiro Tomoda, and Shinya Yamanaka,
Cell, 131(5):861-72, 30 November 2007.
[Authors' affiliations: Kyoto University, Japan; CREST, Kawaguchi, Japan;
Gladstone Institute of Cardiovascular Disease, San Francisco, CA]
Abstract: "Successful reprogramming of differentiated
human somatic cells into a pluripotent state would allow creation of
patient- and disease-specific stem cells. We previously reported generation
of induced pluripotent stem (iPS) cells, capable of germline transmission,
from mouse somatic cells by transduction of four defined transcription
factors. Here, we demonstrate the generation of iPS cells from adult human
dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and
c-Myc. Human iPS cells were similar to human embryonic stem (ES) cells in
morphology, proliferation, surface antigens, gene expression, epigenetic
status of pluripotent cell-specific genes, and telomerase activity.
Furthermore, these cells could differentiate into cell types of the three
germ layers in vitro and in teratomas. These findings demonstrate that iPS
cells can be generated from adult human fibroblasts.
This 2007 report from Cell was cited 41
times in current journal articles indexed by Thomson Reuters
during March-April 2008. During that two-month period, only one other
biology paper published in the last two years, aside from reviews,
attracted a higher number of citations. Prior to the most recent bimonthly
count, citations to the paper have accrued as follows:
January-February 2008: 23 citations
November-December 2007: 3
Total citations to date: 67
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