Hot Paper in Biology

January 2014
“The Accessible Chromatin Landscape of the Human Genome,” by R.E. Thurman, et al., Nature, 489(7414): 75-82, 6 September 2012.

Abstract: “DNase I hypersensitive sites (DHSs) are markers of regulatory DNA and have underpinned the discovery of all classes of cis-regulatory elements including enhancers, promoters, insulators, silencers and locus control regions. Here we present the first extensive map of human DHSs identified through genome-wide profiling in 125 diverse cell and tissue types. We identify similar to 2.9 million DHSs that encompass virtually all known experimentally validated cis-regulatory sequences and expose a vast trove of novel elements, most with highly cell-selective regulation. Annotating these elements using ENCODE data reveals novel relationships between chromatin accessibility, transcription, DNA methylation and regulatory factor occupancy patterns. We connect similar to 580,000 distal DHSs with their target promoters, revealing systematic pairing of different classes of distal DHSs and specific promoter types. Patterning of chromatin accessibility at many regulatory regions is organized with dozens to hundreds of co-activated elements, and the transcellular DNase I sensitivity pattern at a given region can predict cell-type-specific functional behaviours. The DHS landscape shows signatures of recent functional evolutionary constraint. However, the DHS compartment in pluripotent and immortalized cells exhibits higher mutation rates than that in highly differentiated cells, exposing an unexpected link between chromatin accessibility, proliferative potential and patterns of human variation.”

This 2012 report from Nature was cited 50 times in current journal articles indexed by Thomson Reuters during July-August 2013. With its latest two-month count, the paper currently stands as the second-most-cited biology paper published in the last two years, aside from reviews (and surpassed only by another paper in the same issue of Nature, one of six adjoining reports in that issue examining functional elements in the human genome, based on data compiled by the ENCODE Project). Prior to the most recent count, citations to the report have accrued as follows, as tracked by Essential Science Indicators Hot Papers:

May-June 2013: 18 citations
March-April 2013: 18
January-February 2013: 5
November-December 2012: 2
September-October 2012: 5

Total citations to date: 98

SOURCE: Thomson Reuters Web of Science

The data and citation records included in this report are from Thomson Reuters Web of ScienceTM. Web of ScienceTM is a registered trademark of Thomson Reuters. All rights reserved.