Hot Paper in Biology

February 2015
“Multiplex genome engineering using CRISPR/Cas systems” by Le Cong, et al., Science, 339(6121): 819-23, 15 February 2013.

Abstract: “Functional elucidation of causal genetic variants and elements requires precise genome editing technologies. The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage. We engineered two different type II CRISPR/Cas systems and demonstrate that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells. Cas9 can also be converted into a nicking enzyme to facilitate homology-directed repair with minimal mutagenic activity. Lastly, multiple guide sequences can be encoded into a single CRISPR array to enable simultaneous editing of several sites within the mammalian genome, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.”


This 2013 report from Science was cited 73 times in current journal articles indexed by Thomson Reuters during September-October 2014. Only one other Biology paper published in the last two years (excluding reviews) recorded a higher number of citations during that two-month period. Prior to the most recent bimonthly count, citations to the report have accrued as follows, as tracked by Essential Science Indicators Hot Papers:

July-August 2014: 82 citations
May-June 2014: 88
March-April 2014: 76
January-February 2014: 94
November-December 2013: 57
September-October 2013: 52
July-August 2013: 34
May-June 2013: 18
March-April 2013: 12
January-February 2013: 17


Total citations to date: 603


SOURCE: Thomson Reuters Web of Science

The data and citation records included in this report are from Thomson Reuters Web of ScienceTM. Web of ScienceTM is a registered trademark of Thomson Reuters. All rights reserved.