Hot Paper in Biology

August 2014
“Multiplex genome engineering using CRISPR/Cas systems” by Le Cong and colleagues, Science, 339(6121): 819-23, 15 February 2013.

[Authors' affiliations: Broad Institute, Cambridge, MA; MIT, Cambridge, MA; Harvard University, Cambridge, MA, Tsinghua University, Beijing, China; Columbia University, New York, NY; Rockefeller University, New York, NY]

Abstract: “Functional elucidation of causal genetic variants and elements requires precise genome editing technologies. The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage. We engineered two different type II CRISPR/Cas systems and demonstrate that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells. Cas9 can also be converted into a nicking enzyme to facilitate homology-directed repair with minimal mutagenic activity. Lastly, multiple guide sequences can be encoded into a single CRISPR array to enable simultaneous editing of several sites within the mammalian genome, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.”


This 2013 report from Science was cited 68 times in current journal articles indexed by Thomson Reuters during March-April 2014. During that two-month period, only two other Biology papers (aside from reviews) collected higher numbers of citations. Prior to the most recent bimonthly count, citations to the report have accrued as follows, as tracked by Essential Science Indicators Hot Papers:


January-February 2014: 75 citations
November-December 2013: 56
September-October 2013: 52
July-August 2013: 34
May-June 2013: 18
March-April 2013: 12
January-February 2013: 17


Total citations to date: 332


SOURCE: Thomson Reuters Web of Science

The data and citation records included in this report are from Thomson Reuters Web of ScienceTM. Web of ScienceTM is a registered trademark of Thomson Reuters. All rights reserved.